Xenobiotic Nucleic Acid (XNA) Synthesis by Phi29 DNA Polymerase
نویسندگان
چکیده
منابع مشابه
A DNA nanotransport device powered by polymerase phi29.
Polymerases are a family of enzymes responsible for copying or replication of nucleic acids (DNA or RNA) templates and hence sustenance of life processes. In this paper, we present a method to exploit a strand-displacing polymerase phi29 as a driving force for nanoscale transportation devices. The principal idea behind the device is strong strand displacement ability of phi29, which can displac...
متن کاملProcessive replication of single DNA molecules in a nanopore catalyzed by phi29 DNA polymerase.
Coupling nucleic acid processing enzymes to nanoscale pores allows controlled movement of individual DNA or RNA strands that is reported as an ionic current/time series. Hundreds of individual enzyme complexes can be examined in single-file order at high bandwidth and spatial resolution. The bacteriophage phi29 DNA polymerase (phi29 DNAP) is an attractive candidate for this technology, due to i...
متن کاملNucleic Acid Synthesis
INTRODUCTION Synthesis in the field of nucleic acids has been a major and continuing interest in my laboratory ever since we knew the structures of the nucleic acids. Clearly the foundations of synthetic work in the nucleotide field were laid by the prolonged and intensive efforts of Professor Todd's laboratory (Cambridge, England) and it was my good fortune to be associated with this laborator...
متن کاملSynthesis of Neomycin-DNA/ Peptide Nucleic Acid Conjugates
Aminoglycoside antibiotics (Figs. 1 and 2), containing a unique polyamine/ carbohydrate structure, have attracted considerable attention because of their specific interactions with RNA. Their antibacterial activity is mainly exerted by their binding to the RNA of the bacterial ribosome, and thus interfering with the mRNA translation process. The miscoding causes membrane damage, which eventuall...
متن کاملThe inhibition of nucleic acid synthesis by mycophenolic acid.
1. Mycophenolic acid, an antibiotic of some antiquity that more recently has been found to have marked activity against a range of tumours in mice and rats, strongly inhibits DNA synthesis in the L strain of fibroblasts in vitro. 2. The extent of the inhibition of DNA synthesis is markedly increased by preincubation of the cells with mycophenolic acid before the addition of [(14)C]thymidine. 3....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Protocols in Chemical Biology
سال: 2018
ISSN: 2160-4762
DOI: 10.1002/cpch.41